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yclJ [2018-10-10 18:53:39]
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yclJ [2018-10-10 18:53:39]

two-component response regulator (OmpR family), active under anaerobic conditions
locus
BSU03750
pI
4.58
mw
26.36 kDa
protein length
227 aa Sequence Blast
gene length
681 bp Sequence Blast
function
unknown
product
two-component response regulator (OmpR family)
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.1|Two-component system response regulators]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.2|Phosphorylation on an Asp residue]

    • Gene

    Coordinates
    426,577 → 427,260

    The protein

    Protein family

  • [SW|OmpR family] of two-component response regulators

    • Paralogous protein(s)

  • [protein|7F340423A34CE40D1F1AA8D373F7C4B859A6496D|WalR], [protein|6A37531896205A9B894C81AB0563C216C0B52CD7|YkoG], [protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]

    • Modification

  • phosphorylated by [protein|52BB660B0CAB36C74F1D47963235846B45AF78AB|YclK] on an Asp residue

    • Effectors of protein activity

  • phosphorylation likely affects DNA-binding activity

    • Structure

  • [PDB|2OQR] (RegX3 from Mycobacterium tuberculosis, 42% identity) [pubmed|17942407]

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    genes
    [gene|706983E6942E883D3A9D45693E7B4015AEABE60B|yclJ]-[gene|52BB660B0CAB36C74F1D47963235846B45AF78AB|yclK]
    description
    [Pubmed|15375128]

    regulatory mechanism

  • [protein|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD]: activation, [Pubmed|15375128], in [regulon|1E829D639BADDC6BDC9B5FB4C97A632A53FDDB24|ResD regulon]

    • regulation

  • expressed under anaerobic conditions ([protein|search|ResD]) [Pubmed|15375128]
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    Biological materials

    Mutant

  • MGNA-C007 (yclJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2005 NBRP B. subtilis, Japan]
  • BKE03750 (Δ[gene|706983E6942E883D3A9D45693E7B4015AEABE60B|yclJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATATCCTCCGGTTGTT, downstream forward: _UP4_ACTGTGTGGGGAGTAGGGTA
  • BKK03750 (Δ[gene|706983E6942E883D3A9D45693E7B4015AEABE60B|yclJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTATATCCTCCGGTTGTT, downstream forward: _UP4_ACTGTGTGGGGAGTAGGGTA

    • References

  • 10094672,20512483,15375128,23504016,17942407